We are using mass spectrometry coupled to chromatographic separation for metabolomics.

The following instruments are in our facility:

  1. GC-Q-MS (Agilent 7890 GC coupled 5975 single quadrupole MS)
  2. GC-QQQ-MS (Agilent 7890 GC coupled to 7000 triple quadrupole MS)
  3. LC-QTOF-MS (Agilent 1200 series LC coupled to 6520 QTOF MS)
  4. LC-QQQ-MS (Agilent 1200 series LC coupled to 6420 triple quadrupole MS)
  5. LC-QQQ-MS (Agilent 1290 LC coupled to 6490 triple quadrupole MS)
  6. LC-QTRAP-MS (Shimadzu LC coupled AB Sciex 4000 QTRAP-MS)
  7. MALDI-FT-ICR-MS (Bruker SolariX 7T FT-ICR-MS with a MALDI and ESI source)

The following methods are used for our research:
1. GC-MS based metabolite profiling using GC-Q-MS
2. GC-MS based fatty acid profiling using GC-Q-MS
3. GC-MS based metabolite quantification using GC-QQQ-MS
4. LC-MS based metabolite profiling using LC-QTOF-MS
5. LC-MS based metabolite quantification using LC-QQQ-MS
6. MALDI based metabolite and lipid imaging using FT-ICR-MS
7. MALDI based lipid analysis using TLC-MALDI-FT-ICR-MS
8. Metabolite identification using MS, MS/MS and MSn